IgE epitope proximity determines immune complex shape and effector cell activation capacity.

ABSTRACT:

Background

IgE-allergen complexes induce mast cell and basophil activation and thus immediate allergic inflammation. They are also important for IgE-facilitated allergen presentation to T cells by antigen-presenting cells.

Objective

To investigate whether the proximity of IgE binding sites on an allergen affects immune complex shape and subsequent effector cell activation in vitro and in vivo.

Methods

We constructed artificial allergens by grafting IgE epitopes in different numbers and proximity onto a scaffold protein. The shape of immune complexes formed between artificial allergens and the corresponding IgE was studied by negative-stain electron microscopy. Allergenic activity was determined using basophil activation assays. Mice were primed with IgE, followed by i

SUBMITTER: Gieras A 

PROVIDER: S-EPMC4890651 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature