An inverse small molecule screen to design a chemically defined medium supporting long-term growth of Drosophila cell lines.

ABSTRACT: Drosophila cell culture is used as a model system with multiple applications including the identification of new therapeutic targets in screens, the study of conserved signal transduction pathway mechanisms, and as an expression system for recombinant proteins. However, in vitro methods for Drosophila cell and organ cultures are relatively undeveloped. To characterize the minimal requirements for long-term maintenance of Drosophila cell lines, we developed an inverse screening strategy to identify small molecules and synergies stimulating proliferation in a chemically defined medium. In this chemical-genetics approach, a compound-protein interaction database is used to systematically score genetic targets on a screen-wide scale to extract further information about cell growth. In the pilot

SUBMITTER: Burnette M 

PROVIDER: S-EPMC4890965 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature