Chromothripsis-like chromosomal rearrangements induced by ionizing radiation using proton microbeam irradiation system.

ABSTRACT: Chromothripsis is the massive but highly localized chromosomal rearrangement in response to a one-step catastrophic event, rather than an accumulation of a series of subsequent and random alterations. Chromothripsis occurs commonly in various human cancers and is thought to be associated with increased malignancy and carcinogenesis. However, the causes and consequences of chromothripsis remain unclear. Therefore, to identify the mechanism underlying the generation of chromothripsis, we investigated whether chromothripsis could be artificially induced by ionizing radiation. We first elicited DNA double-strand breaks in an oral squamous cell carcinoma cell line HOC313-P and its highly metastatic subline HOC313-LM, using Single Particle Irradiation system to Cell (SPICE), a focused vertical m

SUBMITTER: Morishita M 

PROVIDER: S-EPMC4891112 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature