Renal Tubular Dysfunction in Pediatric Urolithiasis: Proteomic Evidence.

ABSTRACT:

Objective

To investigate whether inflammation, oxidation, and tubular injury are present in children with stones (RS) compared with healthy controls (HC) by measuring urinary proteins involved in these processes.

Methods

Quantitative proteomic comparison of pooled urine from RS (N = 30, 24 females, mean age 12.95 ± 4.03 years) versus age- and gender-matched HC (N = 30), using liquid chromatography-mass spectrometry. Relative protein abundance was estimated using spectral counting. Proteins of interest were selected using the following criteria: (1) ≥5 spectral counts; (2) ≥2-fold difference in spectral counts; and (3) P-value ≤.05 for Fisher's exact test.

Results

Of the 1813 proteins identified, 230 met the above criteria, with 163 proteins upregulated in the RS group

SUBMITTER: Kovacevic L 

PROVIDER: S-EPMC4891186 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature