OTUB1 promotes tumor invasion and predicts a poor prognosis in gastric adenocarcinoma.

ABSTRACT:

Backgrounds

The deubiquitinating enzyme OTUB1 participates in multiple cellular processes. However, its expression and functions in gastric adenocarcinoma remains unknown. The aim of this study was to investigate the expression of OTUB1 and its biological role in gastric adenocarcinoma.

Methods

We used immunohistochemistry to analyze OTUB1 expressions levels in 80 paired samples of gastric adenocarcinoma and adjacent normal tissue (ANT) and 30 samples of intraepithelial neoplasia (IN). We also analyzed the correlation between OTUB1 expression and clinicopathological parameters and patient survival status. Moreover, we performed wound-healing, transwell, RT-qPCR and Western blot assays to evaluate the impact of OTUB1 on tumor migration and invasion.

Results

In gastric

SUBMITTER: Weng W 

PROVIDER: S-EPMC4891435 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature