Full-length LGR5-positive cells have chemoresistant characteristics in colorectal cancer.

ABSTRACT:

Background

Leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) is a target of Wnt signalling and considered both a cancer stem cell marker and intestinal stem cell marker. We found first some splice variants of LGR5 in human intestine and elucidated the functional feature of full-length LGR5 (LGR5FL).

Methods

Reverse transcript PCR using mRNA extracted from intestine revealed the existence of LGR5 splice variants. We designed an antibody that recognises only LGR5FL and assessed immunohistochemically the distribution of LGR5FL-positive cells and Ki-67-positive cells in clinical samples.

Results

Two LGR5 splice variants were expressed in the human intestine crypt cells; one lacked exon 5 and the other lacked exons 5-8. Only LGR5FL appeared during cell cyc

SUBMITTER: Osawa H 

PROVIDER: S-EPMC4891500 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature