Circulatory shear flow alters the viability and proliferation of circulating colon cancer cells.

ABSTRACT: During cancer metastasis, circulating tumor cells constantly experience hemodynamic shear stress in the circulation. Cellular responses to shear stress including cell viability and proliferation thus play critical roles in cancer metastasis. Here, we developed a microfluidic approach to establish a circulatory microenvironment and studied circulating human colon cancer HCT116 cells in response to a variety of magnitude of shear stress and circulating time. Our results showed that cell viability decreased with the increase of circulating time, but increased with the magnitude of wall shear stress. Proliferation of cells survived from circulation could be maintained when physiologically relevant wall shear stresses were applied. High wall shear stress (60.5 dyne/cm(2)), however, led to decre

SUBMITTER: Fan R 

PROVIDER: S-EPMC4891768 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature