Use of HCA in subproteome-immunization and screening of hybridoma supernatants to define distinct antibody binding patterns.

ABSTRACT: Understanding the properties and functions of complex biological systems depends upon knowing the proteins present and the interactions between them. Recent advances in mass spectrometry have given us greater insights into the participating proteomes, however, monoclonal antibodies remain key to understanding the structures, functions, locations and macromolecular interactions of the involved proteins. The traditional single immunogen method to produce monoclonal antibodies using hybridoma technology are time, resource and cost intensive, limiting the number of reagents that are available. Using a high content analysis screening approach, we have developed a method in which a complex mixture of proteins (e.g., subproteome) is used to generate a panel of monoclonal antibodies specific to a

SUBMITTER: Szafran AT 

PROVIDER: S-EPMC4891975 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature