Intrathecal T-cell clonal expansions in patients with multiple sclerosis.

ABSTRACT:

Objective

Analysis of the T-cell receptor (TCR) repertoire in the cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) can reveal antigen-specific immune responses potentially implicated in the disease process. We applied a new unbiased deep-sequencing method for TCR repertoire analysis to accurately measure and compare receptor diversity and clonal expansions within the peripheral and CSF-trafficking T-cell populations of patients with MS and control individuals with idiopathic intracranial hypertension (IIH).

Methods

Paired blood and CSF TCR β-chain libraries from five MS patients and five IIH controls were sequenced, yielding a total of 80 million reads.

Results

Although TCR repertoire diversity was greater in the blood and CSF compartments of MS pati

SUBMITTER: de Paula Alves Sousa A 

PROVIDER: S-EPMC4891996 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature