Measurement of Electroretinograms and Visually Evoked Potentials in Awake Moving Mice.

ABSTRACT: The development of new treatments for intractable retinal diseases requires reliable functional assessment tools for animal models. In vivo measurements of neural activity within visual pathways, including electroretinogram (ERG) and visually evoked potential (VEP) recordings, are commonly used for such purposes. In mice, the ERG and VEPs are usually recorded under general anesthesia, a state that may alter sensory transduction and neurotransmission, but seldom in awake freely moving mice. Therefore, it remains unknown whether the electrophysiological assessment of anesthetized mice accurately reflects the physiological function of the visual pathway. Herein, we describe a novel method to record the ERG and VEPs simultaneously in freely moving mice by immobilizing the head using a custom-b

SUBMITTER: Tomiyama Y 

PROVIDER: S-EPMC4892628 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature