Intratumoral Heterogeneity of MicroRNA Expression in Rectal Cancer.

ABSTRACT:

Introduction

An increasing number of studies have investigated microRNAs (miRNAs) as potential markers of diagnosis, treatment and prognosis. So far, agreement between studies has been minimal, which may in part be explained by intratumoral heterogeneity of miRNA expression. The aim of the present study was to assess the heterogeneity of a panel of selected miRNAs in rectal cancer, using two different technical approaches.

Materials and methods

The expression of the investigated miRNAs was analysed by real-time quantitative polymerase chain reaction (RT-qPCR) and in situ hybridization (ISH) in tumour specimens from 27 patients with T3-4 rectal cancer. From each tumour, tissue from three different luminal localisations was examined. Inter- and intra-patient variability was as

SUBMITTER: Eriksen AH 

PROVIDER: S-EPMC4892647 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature