Short-Term Differentiation of Glioblastoma Stem Cells Induces Hypoxia Tolerance.

ABSTRACT: Glioblastoma is the most common and malignant brain cancer. In spite of surgical removal, radiation and chemotherapy, this cancer recurs within short time and median survival after diagnosis is less than a year. Glioblastoma stem cells (GSCs) left in the brain after surgery is thought to explain the inevitable recurrence of the tumor. Although hypoxia is a prime factor contributing to treatment resistance in many cancers, its effect on GSC has been little studied. Especially how differentiation influences the tolerance to acute hypoxia in GSCs is not well explored. We cultured GSCs from three patient biopsies and exposed these and their differentiated (1- and 4-weeks) progeny to acute hypoxia while monitoring intracellular calcium and mitochondrial membrane potential (ΔΨm). Undifferentiate

SUBMITTER: Skjellegrind HK 

PROVIDER: S-EPMC4893075 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature