IgG is involved in the migration and invasion of clear cell renal cell carcinoma.

ABSTRACT:

Objective

To investigate if IgG can be expressed in clear cell renal cell carcinoma (cRCC) , and the expression of IgG is involved in the cancer progression. If IgG expression can serve as a potential target in cancer therapies and be used for judging the prognosis.

Materials and methods

By immunohistochemistry, we detected IgG in cRCC tissues(75 cRCC tissues and75 adjacent normal kidney tissues). Immunofluorescence and Western blot was used to detect the IgG in cRCC cell lines (786-0, ACHN and CAKI-I). By RT-PCR, the functional transcript of IgG heavy chain was detected. Knockdown of IgG was to analyze the proliferation, migration and invasion ability by CCK8, Transwell and Matrigel and apoptosis in cRCC cell lines.

Results

By immunohistochemistry, we found strong st

SUBMITTER: Sheng Z 

PROVIDER: S-EPMC4893138 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature