Functional Properties of Dendritic Gap Junctions in Cerebellar Golgi Cells.

ABSTRACT: The strength and variability of electrical synaptic connections between GABAergic interneurons are key determinants of spike synchrony within neuronal networks. However, little is known about how electrical coupling strength is determined due to the inaccessibility of gap junctions on the dendritic tree. We investigated the properties of gap junctions in cerebellar interneurons by combining paired somato-somatic and somato-dendritic recordings, anatomical reconstructions, immunohistochemistry, electron microscopy, and modeling. By fitting detailed compartmental models of Golgi cells to their somato-dendritic voltage responses, we determined their passive electrical properties and the mean gap junction conductance (0.9 nS). Connexin36 immunofluorescence and freeze-fracture replica immunogol

SUBMITTER: Szoboszlay M 

PROVIDER: S-EPMC4893164 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature