Comprehensive analysis of promoter-proximal RNA polymerase II pausing across mammalian cell types.

ABSTRACT:

Background

For many genes, RNA polymerase II stably pauses before transitioning to productive elongation. Although polymerase II pausing has been shown to be a mechanism for regulating transcriptional activation, the extent to which it is involved in control of mammalian gene expression and its relationship to chromatin structure remain poorly understood.

Results

Here, we analyze 85 RNA polymerase II chromatin immunoprecipitation (ChIP)-sequencing experiments from 35 different murine and human samples, as well as related genome-wide datasets, to gain new insights into the relationship between polymerase II pausing and gene regulation. Across cell and tissue types, paused genes (pausing index > 2) comprise approximately 60 % of expressed genes and are repeatedly associated wi

SUBMITTER: Day DS 

PROVIDER: S-EPMC4893286 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature