Intratracheal myriocin enhances allergen-induced Th2 inflammation and airway hyper-responsiveness.

ABSTRACT:

Introduction

Ceramide is the central substrate of sphingolipid metabolism and plays a key role in cellular signal transduction pathways, regulating apoptosis, differentiation, and chemotaxis. Alterations in airway ceramide levels are observed in multiple pulmonary diseases and recent human genetic association studies have linked dysregulation of sphingolipid regulatory genes with asthma pathogenesis.

Methods

Utilizing myriocin, a potent inhibitor of sphingolipid synthesis, we evaluated the immune regulatory role of de novo ceramide generation in vitro and in vivo. Intratracheal myriocin was administered alone or during house dust mite sensitization (HDM) of BALB/C mice and airway hyper-responsiveness (AHR) was evaluated by invasive plethysmography followed by bronchial lavag

SUBMITTER: Edukulla R 

PROVIDER: S-EPMC4893390 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature