Synovial fluid proteome in rheumatoid arthritis.

ABSTRACT:

Background

Rheumatoid arthritis (RA) is a chronic autoinflammatory disorder that affects small joints. Despite intense efforts, there are currently no definitive markers for early diagnosis of RA and for monitoring the progression of this disease, though some of the markers like anti CCP antibodies and anti vimentin antibodies are promising. We sought to catalogue the proteins present in the synovial fluid of patients with RA. It was done with the aim of identifying newer biomarkers, if any, that might prove promising in future.

Methods

To enrich the low abundance proteins, we undertook two approaches-multiple affinity removal system (MARS14) to deplete some of the most abundant proteins and lectin affinity chromatography for enrichment of glycoproteins. The peptides were an

SUBMITTER: Bhattacharjee M 

PROVIDER: S-EPMC4893419 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature