An extra dimension in protein tagging by quantifying universal proteotypic peptides using targeted proteomics.

ABSTRACT: The use of protein tagging to facilitate detailed characterization of target proteins has not only revolutionized cell biology, but also enabled biochemical analysis through efficient recovery of the protein complexes wherein the tagged proteins reside. The endogenous use of these tags for detailed protein characterization is widespread in lower organisms that allow for efficient homologous recombination. With the recent advances in genome engineering, tagging of endogenous proteins is now within reach for most experimental systems, including mammalian cell lines cultures. In this work, we describe the selection of peptides with ideal mass spectrometry characteristics for use in quantification of tagged proteins using targeted proteomics. We mined the proteome of the hyperthermophile Pyroc

SUBMITTER: Vandemoortele G 

PROVIDER: S-EPMC4893672 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature