Profiling status epilepticus-induced changes in hippocampal RNA expression using high-throughput RNA sequencing.

ABSTRACT: Status epilepticus (SE) is a life-threatening condition that can give rise to a number of neurological disorders, including learning deficits, depression, and epilepsy. Many of the effects of SE appear to be mediated by alterations in gene expression. To gain deeper insight into how SE affects the transcriptome, we employed the pilocarpine SE model in mice and Illumina-based high-throughput sequencing to characterize alterations in gene expression from the induction of SE, to the development of spontaneous seizure activity. While some genes were upregulated over the entire course of the pathological progression, each of the three sequenced time points (12-hour, 10-days and 6-weeks post-SE) had a largely unique transcriptional profile. Hence, genes that regulate synaptic physiology and tran

SUBMITTER: Hansen KF 

PROVIDER: S-EPMC4894418 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature