Analysis of protein phosphorylation in nerve terminal reveals extensive changes in active zone proteins upon exocytosis.

ABSTRACT: Neurotransmitter release is mediated by the fast, calcium-triggered fusion of synaptic vesicles with the presynaptic plasma membrane, followed by endocytosis and recycling of the membrane of synaptic vesicles. While many of the proteins governing these processes are known, their regulation is only beginning to be understood. Here we have applied quantitative phosphoproteomics to identify changes in phosphorylation status of presynaptic proteins in resting and stimulated nerve terminals isolated from the brains of Wistar rats. Using rigorous quantification, we identified 252 phosphosites that are either up- or downregulated upon triggering calcium-dependent exocytosis. Particularly pronounced were regulated changes of phosphosites within protein constituents of the presynaptic active zone,

SUBMITTER: Kohansal-Nodehi M 

PROVIDER: S-EPMC4894758 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature