Characterization of DNA methylation and its association with other biological systems in lymphoblastoid cell lines.

ABSTRACT: Lymphoblastoid cell line (LCL) is a common tool to study genetic disorders. However, it has not been fully characterized to what degree LCLs preserve the in vivo status of non-genetic biological systems, such as DNA methylation and gene transcription. We previously reported that DNA methylation in LCLs is highly variable in a data set of ~27,000 CpG dinucleotide sites around transcription start site (TSS) and 63 human subjects including healthy controls and probands of genetic disorders. Disease-causing mutations are linked to differential methylation at some CpG sites, but account for a small proportion of the total variance. In this study, we repeated the experiments to ensure that the high variance is not due to technical error and scrutinized the characteristics of DNA methylation and

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC4894836 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature