Klf2a couples mechanotransduction and zebrafish valve morphogenesis through fibronectin synthesis.

ABSTRACT: The heartbeat and blood flow signal to endocardial cell progenitors through mechanosensitive proteins that modulate the genetic program controlling heart valve morphogenesis. To date, the mechanism by which mechanical forces coordinate tissue morphogenesis is poorly understood. Here we use high-resolution imaging to uncover the coordinated cell behaviours leading to heart valve formation. We find that heart valves originate from progenitors located in the ventricle and atrium that generate the valve leaflets through a coordinated set of endocardial tissue movements. Gene profiling analyses and live imaging reveal that this reorganization is dependent on extracellular matrix proteins, in particular on the expression of fibronectin1b. We show that blood flow and klf2a, a major endocardial fl

SUBMITTER: Steed E 

PROVIDER: S-EPMC4894956 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature