Hepatocellular carcinoma cell lines retain the genomic and transcriptomic landscapes of primary human cancers.

ABSTRACT: Hepatocellular carcinoma (HCC) cell lines are useful in vitro models for the study of primary HCCs. Because cell lines acquire additional mutations in culture, it is important to understand to what extent HCC cell lines retain the genetic landscapes of primary HCCs. Most HCC cell lines were established during the last century, precluding comparison between cell lines and primary cancers. In this study, 9 Chinese HCC cell lines with matched patient-derived cells at low passages (PDCs) were established in the defined culture condition. Whole genome analyses of 4 HCC cell lines showed that genomic mutation landscapes, including mutations, copy number alterations (CNAs) and HBV integrations, were highly stable during cell line establishment. Importantly, genetic alterations in cancer drivers a

SUBMITTER: Qiu Z 

PROVIDER: S-EPMC4895220 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature