A new scheme to discover functional associations and regulatory networks of E3 ubiquitin ligases.

ABSTRACT:

Background

Protein ubiquitination catalyzed by E3 ubiquitin ligases play important modulatory roles in various biological processes. With the emergence of high-throughput mass spectrometry technology, the proteomics research community embraced the development of numerous experimental methods for the determination of ubiquitination sites. The result is an accumulation of ubiquitinome data, coupled with a lack of available resources for investigating the regulatory networks among E3 ligases and ubiquitinated proteins. In this study, by integrating existing ubiquitinome data, experimentally validated E3 ligases and established protein-protein interactions, we have devised a strategy to construct a comprehensive map of protein ubiquitination networks.

Results

In total, 41,392 ex

SUBMITTER: Huang KY 

PROVIDER: S-EPMC4895279 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature