TALEN-mediated gene editing of the thrombospondin-1 locus in axolotl.

ABSTRACT: Loss-of-function genetics provides strong evidence for a gene's function in a wild-type context. In many model systems, this approach has been invaluable for discovering the function of genes in diverse biological processes. Axolotls are urodele amphibians (salamanders) with astonishing regenerative abilities, capable of regenerating entire limbs, portions of the tail (including spinal cord), heart, and brain into adulthood. With their relatively short generation time among salamanders, they offer an outstanding opportunity to interrogate natural mechanisms for appendage and organ regeneration provided that the tools are developed to address these long-standing questions. Here we demonstrate targeted modification of the thrombospondin-1 (tsp-1) locus using transcription-activator-like effe

SUBMITTER: Kuo TH 

PROVIDER: S-EPMC4895330 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature