Transcriptome sequencing based annotation and homologous evidence based scaffolding of Anguilla japonica draft genome.

ABSTRACT:

Background

Anguilla japonica (Japanese eel) is currently one of the most important research subjects in eastern Asia aquaculture. Enigmatic life cycle of the organism makes study of artificial reproduction extremely limited. Henceforth genomic and transcriptomic resources of eels are urgently needed to help solving the problems surrounding this organism across multiple fields. We hereby provide a reconstructed transcriptome from deep sequencing of juvenile (glass eels) whole body samples. The provided expressed sequence tags were used to annotate the currently available draft genome sequence. Homologous information derived from the annotation result was applied to improve the group of scaffolds into available linkage groups.

Results

With the transcriptome sequence data combi

SUBMITTER: Liu YC 

PROVIDER: S-EPMC4895481 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature