Measurement of IL-12 (p40, p35), IL-23p19, and IFN-γ mRNA in duodenal biopsies of cats with inflammatory enteropathy.

ABSTRACT:

Background

Dietary hypersensitivity and inflammatory bowel disease (IBD) are important causes of chronic vomiting and diarrhea in cats. IL-23 has been recently found to be a key factor in the immunopathogenesis of IBD in humans but the involvement in IBD has not been investigated in cats.

Hypothesis/objectives

Expression of genes encoding Il-12p35 and p40, IL-23p19, and IFN-γ may be up-regulated in duodenal biopsy specimens taken from cats with histologic evidence of inflammation.

Animals and methods

Duodenal biopsy specimens were collected from control cats (n = 21) and cats with inflammatory enteropathy (n = 13). Routine histopathology, immunohistochemistry (IHC), and qRT-PCR were used to assess expression of MHC class II and to measure gene transcripts encoding the

SUBMITTER: Waly NE 

PROVIDER: S-EPMC4895550 | biostudies-literature | 2014 Jan-Feb

REPOSITORIES: biostudies-literature