Cyclooxygenase-2 and 5-lipoxygenase in dogs with chronic enteropathies.

ABSTRACT:

Background

Cyclooxygenase-2 (COX-2) is a key enzyme in the synthesis of pro-inflammatory prostaglandins and 5-lipoxygenase (5-LO) is the major source of leukotrienes. Their role in IBD has been demonstrated in humans and animal models, but not in dogs with chronic enteropathies (CCE).

Hypothesis

COX-2 and 5-LO are upregulated in dogs with CCE.

Animals

Fifteen healthy control dogs (HCD), 10 dogs with inflammatory bowel disease (IBD), and 15 dogs with food-responsive diarrhea (FRD).

Methods

Prospective study. mRNA expression of COX-2, 5-LO, IL-1b, IL-4, IL-6, TNF, IL-10 and TFG-β was evaluated by quantitative real-time RT-PCR in duodenal and colonic biopsies before and after treatment.

Results

COX-2 expression in the colon was significantly higher in IBD a

SUBMITTER: Dumusc SD 

PROVIDER: S-EPMC4895633 | biostudies-literature | 2014 Nov-Dec

REPOSITORIES: biostudies-literature