Rapid identification of fungi in culture-negative clinical blood and respiratory samples by DNA sequence analyses.

ABSTRACT:

Background

Clinical diagnoses of fungal infections often rely upon culture techniques followed by microscopic examination of positive cultures and histopathological specimens. Culturing of microorganisms is prone to false negatives, while microscopy methods can be complicated by atypical phenotypes and organisms that are morphologically indistinguishable in tissues. Delays in diagnoses (or the lack thereof) and inaccurate identification of infectious organisms contribute to increased morbidity and mortality in patients.

Methods

Two-hundred randomized, heterogeneous patient blood and respiratory samples that were culture-negative were tested using polymerase chain reaction (PCR) amplification of internal transcribed spacer regions of ribosomal RNA genes utilizing panfungal pr

SUBMITTER: Sidiq F 

PROVIDER: S-EPMC4895986 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature