Roles of acid-extruding ion transporters in regulation of breast cancer cell growth in a 3-dimensional microenvironment.

ABSTRACT:

Background

The 3-dimensional (3D) microenvironment of breast carcinomas is characterized by profoundly altered pH homeostasis, reflecting increased metabolic acid production and a confined extracellular space characterized by poor diffusion, yet the relative contributions of specific pH-regulatory transporters to 3D growth are poorly understood. The aim of this work was to determine how 3D spheroid growth of breast cancer cells impacts the expression and spatial organization of major acid extruding proteins, and how these proteins in turn are required for spheroid growth.

Methods

MCF-7 (Luminal-A) and MDA-MB-231 (Triple-negative) human breast cancer cells were grown as ~700-950 μm diameter spheroids, which were subjected to Western blotting for relevant transporters (2- and

SUBMITTER: Andersen AP 

PROVIDER: S-EPMC4896021 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature