Transcript Isoform Variation Associated with Cytosine Modification in Human Lymphoblastoid Cell Lines.

ABSTRACT: Cytosine modification on DNA is variable among individuals, which could correlate with gene expression variation. The effect of cytosine modification on interindividual transcript isoform variation (TIV), however, remains unclear. In this study, we assessed the extent of cytosine modification-specific TIV in lymphoblastoid cell lines (LCLs) derived from unrelated individuals of European and African descent. Our study detected cytosine modification-specific TIVs for 17% of the analyzed genes at a 5% false discovery rate. Forty-five percent of the TIV-associated cytosine modifications correlated with the overall gene expression levels as well, with the corresponding CpG sites overrepresented in transcript initiation sites, transcription factor binding sites, and distinct histone modification

SUBMITTER: Zhang X 

PROVIDER: S-EPMC4896207 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature