Evidence for enhanced collagen type III deposition focally in the territorial matrix of osteoarthritic hip articular cartilage.

ABSTRACT:

Objective

To determine if type III collagen is concentrated in the chymotrypsin-extractable collagen pool from osteoarthritic articular cartilage to assess its potential as a biomarker of Osteoarthritis (OA) pathogenic mechanisms.

Methods

Full thickness articular cartilage from grossly normal surfaces was analyzed from femoral heads, obtained at hip replacement surgery, from OA (n = 10) and fracture (n = 10) patients. Collagen, extracted by α-chymotrypsin, was characterized by SDS-PAGE/Western blot analysis, ELISA and immunohistochemistry using monoclonal antibodies specific to collagens types II and III.

Results

α-Chymotrypsin extracted more collagen from OA than control cartilage. The extractable pool included collagen types II and III from both OA and control hips.

SUBMITTER: Hosseininia S 

PROVIDER: S-EPMC4896400 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature