Metagenomic discovery of novel enzymes and biosurfactants in a slaughterhouse biofilm microbial community.

ABSTRACT: DNA derived from environmental samples is a rich source of novel bioactive molecules. The choice of the habitat to be sampled predefines the properties of the biomolecules to be discovered due to the physiological adaptation of the microbial community to the prevailing environmental conditions. We have constructed a metagenomic library in Escherichia coli DH10b with environmental DNA (eDNA) isolated from the microbial community of a slaughterhouse drain biofilm consisting mainly of species from the family Flavobacteriaceae. By functional screening of this library we have identified several lipases, proteases and two clones (SA343 and SA354) with biosurfactant and hemolytic activities. Sequence analysis of the respective eDNA fragments and subsequent structure homology modelling identified

SUBMITTER: Thies S 

PROVIDER: S-EPMC4897644 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature