Analysis of gene expression in mouse brain regions after exposure to 1.9 GHz radiofrequency fields.

ABSTRACT:

Purpose

To assess 1.9 GHz radiofrequency (RF) field exposure on gene expression within a variety of discrete mouse brain regions using whole genome microarray analysis.

Materials and methods

Adult male C57BL/6 mice were exposed to 1.9 GHz pulse-modulated or continuous-wave RF fields for 4 h/day for 5 consecutive days at whole body average (WBA) specific absorption rates of 0 (sham), ∼0.2 W/kg and ∼1.4 W/kg. Total RNA was isolated from the auditory cortex, amygdala, caudate, cerebellum, hippocampus, hypothalamus, and medial prefrontal cortex and differential gene expression was assessed using Illumina MouseWG-6 (v2) BeadChip arrays. Validation of potentially responding genes was conducted by RT-PCR.

Results

When analysis of gene expression was conducted within individu

SUBMITTER: McNamee JP 

PROVIDER: S-EPMC4898144 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature