Independently inducible system of gene expression for condensed single protein production (cSPP) suitable for high efficiency isotope enrichment.

ABSTRACT: The ability to produce isotope-enriched proteins is fundamental to the success of modern protein NMR, and is particularly essential for NMR activities in structural genomics projects. Conventional methods of protein production often prove to be cost prohibitive for obtaining samples, particularly perdeuterated and site-specifically labeled proteins. The condensed single protein production system (cSPP), providing protein expression following condensation of cells 10-40 fold, allows for the production of such samples at a fraction of the cost. The previously described cSPP system is a two plasmid system where both the MazF toxin and ACA-less target gene are coinduced with IPTG. Coinduction results in 10-20% of the target protein produced without isotopic enrichment. Though the unlabeled pro

SUBMITTER: Schneider WM 

PROVIDER: S-EPMC4898478 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature