Dipeptide-Based Metabolic Labeling of Bacterial Cells for Endogenous Antibody Recruitment.

ABSTRACT: The number of antibiotic-resistant bacterial infections has increased dramatically over the past decade. To combat these pathogens, novel antimicrobial strategies must be explored and developed. We previously reported a strategy based on hapten-modified cell wall analogues to induce recruitment of endogenous antibodies to bacterial cell surfaces. Cell surface remodeling using unnatural single d-amino acid cell wall analogues led to modification at the C-terminus of the peptidoglycan stem peptide. During peptidoglycan processing, installed hapten-displaying amino acids can be subsequently removed by cell wall enzymes. Herein, we disclose a two-step dipeptide peptidoglycan remodeling strategy aimed at introducing haptens at an alternative site within the stem peptide to improve retention and

SUBMITTER: Fura JM 

PROVIDER: S-EPMC4898660 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature