Automated Glycan Sequencing from Tandem Mass Spectra of N-Linked Glycopeptides.

ABSTRACT: Mass spectrometry has become a routine experimental tool for proteomic biomarker analysis of human blood samples, partly due to the large availability of informatics tools. As one of the most common protein post-translational modifications (PTMs) in mammals, protein glycosylation has been observed to alter in multiple human diseases and thus may potentially be candidate markers of disease progression. While mass spectrometry instrumentation has seen advancements in capabilities, discovering glycosylation-related markers using existing software is currently not straightforward. Complete characterization of protein glycosylation requires the identification of intact glycopeptides in samples, including identification of the modification site as well as the structure of the attached glycans. I

SUBMITTER: Yu CY 

PROVIDER: S-EPMC4899231 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature