Ratiometric QD-FRET Sensing of Aqueous H2S in Vitro.

ABSTRACT: We report a platform for the ratiometric fluorescent sensing of endogenously generated gaseous transmitter H2S in its aqueous form (bisulfide or hydrogen sulfide anion) based on the alteration of Förster resonance energy transfer from an emissive semiconductor quantum dot (QD) donor to a dithiol-linked organic dye acceptor. The disulfide bridge between the two chromophores is cleaved upon exposure to bisulfide, resulting in termination of FRET as the dye diffuses away from the QD. This results in enhanced QD emission and dye quenching. The resulting ratiometric response can be correlated quantitatively to the concentration of bisulfide and was found to have a detection limit as low as 1.36 ± 0.03 μM. The potential for use in biological applications was demonstrated by measuring the respons

SUBMITTER: Shamirian A 

PROVIDER: S-EPMC4899257 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature