Differentiation of the Chinese minority medicinal plant genus Berchemia spp. by evaluating three candidate barcodes.

ABSTRACT: The genus Berchemia comprises important Chinese plants with considerable medicinal value; however, these plants are often misidentified in the herbal medicinal market. To differentiate the various morphotypes of Berchemia species, a proficient method employing the screening of universal DNA barcodes was used in this work. Three candidate barcoding loci, namely, psbA-trnH, rbcL, and the second internal transcribed spacer (ITS2), were used to identify an effective DNA barcode that can differentiate the various Berchemia species. Additionally, PCR amplification, efficient sequencing, intra- and inter-specific divergences, and DNA barcoding gaps were employed to assess the ability of each barcode to identify these diverse Berchemia plants authentically; the species were differentiated using th

SUBMITTER: Guo LC 

PROVIDER: S-EPMC4899350 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature