Bioinformatic Analysis of the Human Recombinant Iduronate 2-Sulfate Sulfatase.

ABSTRACT: Mucopolysaccharidosis type II is a human recessive disease linked to the X chromosome caused by deficiency of lysosomal enzyme Iduronate 2-Sulfate Sulfatase (IDS), which leads to accumulation of glycosaminoglycans in tissues and organs. The human enzyme has been expressed in Escherichia coli and Pichia pastoris in attempt to develop more successful expression systems that allow the production of recombinant IDS for Enzyme Replacement Therapy (ERT). However, the preservation of native signal peptide in the sequence has caused conflicts in processing and recognition in the past, which led to problems in expression and enzyme activity. With the main object being the improvement of the expression system, we eliminate the native signal peptide of human recombinant IDS. The resulting sequence sh

SUBMITTER: Morales-Alvarez ED 

PROVIDER: S-EPMC4899537 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature