Identification and Validation of Reference Genes for qRT-PCR Studies of Gene Expression in Dioscorea opposita.

ABSTRACT: Quantitative real-time polymerase chain reaction (qRT-PCR) is one of the most common methods for gene expression studies. Data normalization based on reference genes is essential for obtaining reliable results for qRT-PCR assays. This study evaluated potential reference genes of Chinese yam (Dioscorea opposita Thunb.), which is an important tuber crop and medicinal plant in East Asia. The expression of ten candidate reference genes across 20 samples from different organs and development stages was assessed. We identified the most stable genes for qRT-PCR studies using combined samples from different organs. Our results also suggest that different suitable reference genes or combinations of reference genes for normalization should be applied according to different organs and developmental s

SUBMITTER: Zhao X 

PROVIDER: S-EPMC4899605 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature