Codon optimization of the adenoviral fiber negatively impacts structural protein expression and viral fitness.

ABSTRACT: Codon usage adaptation of lytic viruses to their hosts is determinant for viral fitness. In this work, we analyzed the codon usage of adenoviral proteins by principal component analysis and assessed their codon adaptation to the host. We observed a general clustering of adenoviral proteins according to their function. However, there was a significant variation in the codon preference between the host-interacting fiber protein and the rest of structural late phase proteins, with a non-optimal codon usage of the fiber. To understand the impact of codon bias in the fiber, we optimized the Adenovirus-5 fiber to the codon usage of the hexon structural protein. The optimized fiber displayed increased expression in a non-viral context. However, infection with adenoviruses containing the optimized

SUBMITTER: Villanueva E 

PROVIDER: S-EPMC4899721 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature