Promiscuity in the Enzymatic Catalysis of Phosphate and Sulfate Transfer.

ABSTRACT: The enzymes that facilitate phosphate and sulfate hydrolysis are among the most proficient natural catalysts known to date. Interestingly, a large number of these enzymes are promiscuous catalysts that exhibit both phosphatase and sulfatase activities in the same active site and, on top of that, have also been demonstrated to efficiently catalyze the hydrolysis of other additional substrates with varying degrees of efficiency. Understanding the factors that underlie such multifunctionality is crucial both for understanding functional evolution in enzyme superfamilies and for the development of artificial enzymes. In this Current Topic, we have primarily focused on the structural and mechanistic basis for catalytic promiscuity among enzymes that facilitate both phosphoryl and sulfuryl trans

SUBMITTER: Pabis A 

PROVIDER: S-EPMC4899807 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature