Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude.

ABSTRACT:

Background

In addition to studies of plant gene function and developmental analyses, plant biotechnological use is largely dependent upon transgenic technologies. The moss Physcomitrella patens has become an exciting model system for studying plant molecular processes due to an exceptionally high rate of nuclear gene targeting by homologous recombination compared with other plants. However, its use in transgenic approaches requires expression vectors that incorporate sufficiently strong promoters. To satisfy this requirement, a set of plant expression vectors was constructed and equipped with either heterologous or endogenous promoters.

Results

Promoter activity was quantified using the dual-luciferase reporter assay system. The eight different heterologous promoter construc

SUBMITTER: Horstmann V 

PROVIDER: S-EPMC490084 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature