Differential regulation of serum microRNA expression by HNF1? and HNF1? transcription factors.

ABSTRACT: We aimed to identify microRNAs (miRNAs) under transcriptional control of the HNF1? transcription factor, and investigate whether its effect manifests in serum.The Polish cohort (N?=?60) consisted of 11 patients with HNF1B-MODY, 17 with HNF1A-MODY, 13 with?GCK-MODY, an HbA1c-matched type 1 diabetic group (n?=?9) and ten healthy controls. Replication was performed in 61 clinically-matched British patients mirroring the groups in the Polish cohort. The Polish cohort underwent miRNA serum level profiling with quantitative real-time PCR (qPCR) arrays to identify differentially expressed miRNAs. Validation was performed using qPCR. To determine whether serum content reflects alterations at a cellular level, we quantified miRNA levels in a human hepatocyte cell line (HepG2) with small interfering RNA knockdowns of HNF1? or HNF1?.Significant differences (adjusted p?

SUBMITTER: Fendler W 

PROVIDER: S-EPMC4901123 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature