Large-scale development of SSR markers in tobacco and construction of a linkage map in flue-cured tobacco.

ABSTRACT: Tobacco (Nicotiana tabacum L.), particularly flue-cured tobacco, is one of the most economically important nonfood crops and is also an important model system in plant biotechnology. Despite its importance, only limited molecular marker resources are available for genome analysis, genetic mapping, and breeding. Simple sequence repeats (SSR) are one of the most widely-used molecular markers, having significant advantages including that they are generally co-dominant, easy to use, abundant in eukaryotic organisms, and produce highly reproducible results. In this study, based on the genome sequence data of flue-cured tobacco (K326), we developed a total of 13,645 mostly novel SSR markers, which were working in a set of eighteen tobacco varieties of four different types. A mapping population o

SUBMITTER: Tong Z 

PROVIDER: S-EPMC4902457 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature