Modeling hormonal and inflammatory contributions to preterm and term labor using uterine temporal transcriptomics.

ABSTRACT:

Background

Preterm birth is now recognized as the primary cause of infant mortality worldwide. Interplay between hormonal and inflammatory signaling in the uterus modulates the onset of contractions; however, the relative contribution of each remains unclear. In this study we aimed to characterize temporal transcriptome changes in the uterus preceding term labor and preterm labor (PTL) induced by progesterone withdrawal or inflammation in the mouse and compare these findings with human data.

Methods

Myometrium was collected at multiple time points during gestation and labor from three murine models of parturition: (1) term gestation; (2) PTL induced by RU486; and (3) PTL induced by lipopolysaccharide (LPS). RNA was extracted and cDNA libraries were prepared and sequenced usi

SUBMITTER: Migale R 

PROVIDER: S-EPMC4904357 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature