Study on Rotavirus Infection and Its Genotyping in Children Below 5 Years in South West Iran.

ABSTRACT:

Background

Human rotaviruses are the most important agents for severe dehydrating diarrhea in children below 5 years old. Rotaviruses (RV) is a serious public health problem in developing and developed countries.

Objectives

The aim of this study was to determine the prevalence of rotavirus infection and their genotypes in children younger than 5 years of age with acute diarrhea in Ahvaz, Iran.

Materials and methods

For this study, 200 stool samples from children below 5 years of age with acute diarrhea were collected between October 2011 and March 2012. Initially all stool samples were tested for rotavirus antigen by ELISA, and positive samples were confirmed by RT-PCR targeting the VP6 rotavirus gene. Determination of rotavirus genotypes was carried out by performing

SUBMITTER: Azaran A 

PROVIDER: S-EPMC4904489 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature