Hepatic stellate cell transdifferentiation involves genome-wide remodeling of the DNA methylation landscape.

ABSTRACT:

Background & aims

DNA methylation (5-mC) is an epigenetic mark that is an established regulator of transcriptional repression with an important role in liver fibrosis. Currently, there is very little knowledge available as to how DNA methylation controls the phenotype of hepatic stellate cell (HSC), the key cell type responsible for onset and progression of liver fibrosis. Moreover, recently discovered DNA hydroxymethylation (5-hmC) is involved in transcriptional activation and its patterns are often altered in human diseases. The aim of this study is to investigate the role of DNA methylation/hydroxymethylation in liver fibrosis.

Methods

Levels of 5-mC and 5-hmC were assessed by slot blot in a range of animal liver fibrosis models and human liver diseases. Expression levels

SUBMITTER: Page A 

PROVIDER: S-EPMC4904781 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature