Hydrogen Sulfide Contributes to Retinal Neovascularization in Ischemia-Induced Retinopathy.

ABSTRACT:

Purpose

Hydrogen sulfide (H2S) is an endogenous gaseous signaling molecule with significant pathophysiological importance, but its role in retinal neovascular diseases is unknown. Hydrogen sulfide is generated from L-cysteine by cystathionine-β-synthase (CBS), cystathionine-γ-lyase (CSE), and/or 3-mercaptopyruvate sulfurtransferase (3-MST). The aim of this study was to investigate the role of H2S in retinal neovascularization (NV) in ischemia-induced retinopathy.

Methods

Studies were performed in a murine model of oxygen-induced retinopathy (OIR). Hydrogen sulfide was detected with a fluorescent assay. Western blots and immunohistochemistry were used to assess the changes of H2S-producing enzymes. Gene deletion and pharmacologic inhibition were used to investigate the role o

SUBMITTER: Gersztenkorn D 

PROVIDER: S-EPMC4904802 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature